Enzyme I, general (non sugar-specific) component of the HPr)
function
PTS-dependent sugar transport
product
phosphotransferase system (PTS) enzyme I
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.1|General PTS proteins][category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue][SW|Categories] containing this gene/protein
[SW|phosphotransferase systems], [SW|phosphoproteins], [SW|most abundant proteins]This gene is a member of the following [SW|regulons]
[SW|GlcT regulon], [SW|stringent response]Gene
Coordinates on the chromosome (coding sequence)
1,459,650 -> 1,461,362
The protein
Catalyzed reaction/ biological activity
Phosphoenolpyruvate protein L-histidine = pyruvate protein N(pi)-phospho-L-histidine (according to Swiss-Prot) PEP-dependent autophosphorylation on His-189, transfer of the phosphoryl group to [PtsH|search|HPr] (His-15)Protein family
PEP-utilizing enzyme family (according to Swiss-Prot) PEP-utilizing enzyme family[SW|Domains]
HPr binding site (N-Terminal Domain)pyruvate binding site (C-Terminal Domain)pyrophosphate/phosphate carrier histidine (central Domain)Modification
transient autophosphorylation on His-189''in vivo'' also phosphorylated on Ser-34 or Ser-36 [Pubmed|17218307][SW|Cofactors]
MagnesiumStructure
[http://www.proteopedia.org/wiki/index.php/2wqd 2WQD] (Enzyme I from ''Staphylococcus aureus'') [Pubmed|19801641][SW|Localization]
cytoplasm, even distribution [Pubmed|23475962][SW|Interactions]
[protein|search|PtsI]-[protein|search|PtsH]Expression and Regulation
Operon
''[protein|search|ptsG]-[protein|search|ptsH]-[protein|search|ptsI]'' [Pubmed|11902727]''[protein|search|ptsH]-[protein|search|ptsI]'' [Pubmed|11902727][SW|Sigma factor]
''[protein|search|ptsG]'': [protein|search|SigA] [Pubmed|11902727]''[protein|search|ptsH]'': [protein|search|SigA] [Pubmed|11902727]Regulation
expression activated by glucose (2 fold) ([protein|search|GlcT]) [Pubmed|12850135]the ''[protein|search|ptsH]'' promoter is constitutive [Pubmed|11902727]subject to negative stringent control upon amino acid limitation (due to control of'' [protein|search|ptsG]'' transcription initiation) [Pubmed|20081037]Regulatory mechanism
''[protein|search|ptsG]'': transcriptional antitermination via the [protein|search|GlcT]-dependent [SW|RNA switch] [Pubmed|9765562]Additional information
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]Biological materials
Mutant
available in [SW|Jörg Stülke]'s lab:GP864 (ermC)GP778 (replacement of ''[protein|search|glcT]'' and the ''[protein|search|ptsG]-[protein|search|ptsH]-[protein|search|ptsI]'' operon by a spc cassette), [Pubmed|22722928]Expression vector
pAG3 (His-tag) [Pubmed|9237995], available in [SW|Galinier] labfor expression, purification in ''E. coli'' (His-tag), in [SW|pWH844]: pGP813 available in [SW|Jörg Stülke]'s labLabs working on this gene/protein
[SW|Josef Deutscher], Paris-Grignon, France[SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]References
12850135,17218307,9721285,20081037,19801641,23475962,24129255,15378759 
