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recX [2018-03-08 18:24:27]
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recX [2018-03-08 18:24:27]

recombination protein, modulates the SOS response and facilitates [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA]-mediated recombinational repair and genetic recombination
locus
BSU08520
pI
6.29
mw
30.86 kDa
protein length
264 aa Sequence Blast
gene length
792 bp Sequence Blast
function
[SW|DNA repair/ recombination]
product
recombination protein
essential
no
synonyms
yfhG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

    • Gene

    Coordinates
    925,633 → 926,427

    Phenotypes of a mutant

  • reduced natural transformation with plasmid or chromosomal DNA [Pubmed|23284295]
  • drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) [Pubmed|24285298]

    • The protein

    Catalyzed reaction/ biological activity

  • modulates the "length or packing" of a [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] filament, thereby [protein|144E7D10740C5F146403612B8A0C88E02FB9C572|RecX] facilitates the initiation of recombination and increases recombination across species [Pubmed|23284295]

    • Protein family

  • recX family (according to Swiss-Prot)

    • [SW|Localization]

  • forms foci on the nucleoid upon DNA damage [Pubmed|23284295]
  • forms foci at the cells poles and/ or the nucleoid upon induction of genetic competence [Pubmed|23284295]

    • Expression and Regulation

    Operons

    genes
    [gene|144E7D10740C5F146403612B8A0C88E02FB9C572|recX]-[gene|CEB1A0835C26E14E04BA99F933787C4550A96388|yfhH]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-C361 (yfhG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2359 NBRP B. subtilis, Japan]
  • BKE08520 (Δ[gene|144E7D10740C5F146403612B8A0C88E02FB9C572|recX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTAATCTCCCTTCTCA, downstream forward: _UP4_TTACTGCAGGAAGAGGAGTA
  • BKK08520 (Δ[gene|144E7D10740C5F146403612B8A0C88E02FB9C572|recX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTAATCTCCCTTCTCA, downstream forward: _UP4_TTACTGCAGGAAGAGGAGTA

    • References

  • 23284295,22383849,24285298,24285298