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ygaK
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ygaK

uncharacterized FAD-linked oxidoreductase
locus
BSU_08800
pI
7.12
mw
40.96 kDa
protein length
451 aa Sequence Blast
gene length
1356 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.8|Not yet assigned]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    957,705 959,060

    The protein

    Protein family

  • oxygen-dependent FAD-linked oxidoreductase family (with [protein|23F2385D14DDD5C400DC897E0F0FC814ACFC36B8|YitY] and [protein|C4CC3772CA9FC46D90D2F15AE261106A0BC4453C|CotQ], according to UniProt)

    • Paralogous protein(s)

  • [protein|C4CC3772CA9FC46D90D2F15AE261106A0BC4453C|CotQ]:

    • [SW|Domains]

  • [SW|FAD-binding PCMH-type domain] (aa 29-204) (according to UniProt)

    • [SW|Cofactors]

  • FAD (according to UniProt)

    • Structure

  • [PDB|3W8W] (from ''Streptomyces maritimus'', 33%)

    • Expression and Regulation

    Operons

    genes
    [gene|11499CDC836A40E857B8F5AD746BA2C650DC916B|ygaK]
    description
    [Pubmed|22383849]

    regulation

  • expression during spore [SW|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]
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    Biological materials

    Mutant

  • MGNA-A246 (ygaK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/246 NBRP B. subtilis, Japan]
  • BKE08800 ([gene|11499CDC836A40E857B8F5AD746BA2C650DC916B|ygaK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACTCCCACATCTCTTTC, downstream forward: _UP4_TAAAAAAATGAAGATGGAGC
  • BKK08800 ([gene|11499CDC836A40E857B8F5AD746BA2C650DC916B|ygaK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACTCCCACATCTCTTTC, downstream forward: _UP4_TAAAAAAATGAAGATGGAGC

    • References

  • 22383849,27766092