endo-1,5-alpha-L-arabinosidase
function
arabinan degradation
product
endo-1,5-alpha-L-arabinosidase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.6|Utilization of arabinan/ arabinose/ arabitol][category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins][SW|Categories] containing this gene/protein
[SW|utilization of specific carbon sources]Gene
Coordinates on the chromosome (coding sequence)
4,039,466 -> 4,040,875
The protein
Protein family
glycosyl hydrolase 43 family (according to Swiss-Prot)Kinetic information
With linear-alpha-1,5-l-arabinan as the preferred substrate, the enzyme exhibited an apparent K(m) of 2.0 mg ml(-1) and V(max) of 0.25 mmol min(-1) mg(-1) at pH 7.0 and 50°C. [Pubmed|18408032][SW|Domains]
N-terminal catalytic domain with a characteristic β-propeller fold and a C-terminal domain whose function is unknown [Pubmed|24549757][SW|Cofactors]
Ca2+ [Pubmed|24549757]Structure
[PDB|2X8F] [Pubmed|20883454][SW|Localization]
extracellular (signal peptide) [Pubmed|18957862,18408032]Expression and Regulation
Operon
''abn2'' [Pubmed|18408032][SW|Sigma factor]
[protein|search|SigA], [protein|search|SigH] [Pubmed|18408032]Regulation
repressed by glucose (4.3-fold) ([protein|search|CcpA]) [Pubmed|12850135]expression is stimulated by arabinose and pectin and repressed by glucose [Pubmed|18408032]Regulatory mechanism
[protein|search|CcpA]: transcription repression, unknown [Pubmed|18408032]Labs working on this gene/protein
[http://www.itqb.unl.pt/research/biology/microbial-genetics [Isabel de Sa-Nogueira]], Lisboa, Portugal [homepage]References
18607095,24549757,18408032,18957862,12850135,18408032,20883454 
