3-isopropylmalate dehydratase (small subunit)
function
biosynthesis of leucine
product
3-isopropylmalate dehydratase (small subunit)
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.12|Biosynthesis/ acquisition of branched-chain amino acids][SW|Categories] containing this gene/protein
[SW|biosynthesis/ acquisition of amino acids]This gene is a member of the following [SW|regulons]
[SW|CcpA regulon], [SW|CodY regulon], [SW|FsrA regulon], [SW|T-box], [SW|TnrA regulon]Gene
Coordinates on the chromosome (coding sequence)
2,888,940 -> 2,889,539
The protein
Catalyzed reaction/ biological activity
(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmaleate H2O (according to Swiss-Prot) Protein family
LeuD type 1 subfamily (according to Swiss-Prot)[SW|Cofactors]
contains an iron-sulfur clusterStructure
[PDB|2HCU] (from ''Streptococcus mutans'', 48% identity)Additional information
subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=17981983 PubMed] Expression and Regulation
Operon
''[protein|search|ilvB]-[protein|search|ilvH]-[protein|search|ilvC]-[protein|search|leuA]-[protein|search|leuB]-[protein|search|leuC]-[protein|search|leuD]'' [Pubmed|1577690][SW|Sigma factor]
[protein|search|SigA] [Pubmed|1577690]Regulation
for a complete overview on the regulation of the ilv operon, see [http://www.ncbi.nlm.nih.gov/pubmed/20935095 Brinsmade ''et al''.]repressed by casamino acids [Pubmed|12107147]expression is stimulated in the presence of glucose [Pubmed|12193635]repressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|15547269]repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455]less expressed under conditions of extreme iron limitation ([protein|search|FsrA]) [Pubmed|18697947]Regulatory mechanism
[protein|search|CodY]: transcription repression [Pubmed|12618455][protein|search|CcpA]: transcription activation [Pubmed|12193635]repression by BCAA: tRNA-controlled [SW|RNA switch] ([protein|search|T-box]) that mediates termination/antitermination[protein|search|TnrA]: transcription repression [Pubmed|15547269][protein|search|FsrA]: translational repression [Pubmed|18697947]Additional information
subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=17981983 PubMed] References
15060025,12193635,19258532,18697947,8289305,18641142,11948165,15547269,12618455,15547269,12618455,12107147,25157083,20935095,25755103,24163341,26220295 
