SubtiBank SubtiBank
ykuN
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ykuN

flavodoxin, binds FMN, replaces ferredoxin under conditions of iron limitation, probably involved in electron transfer to nitric oxide synthase
locus
BSU_14150
pI
3.83
mw
17.65 kDa
protein length
158 aa Sequence Blast
gene length
477 bp Sequence Blast
function
electron transfer
product
flavodoxin
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.3|Electron transport/ other]

    • Gene

    Coordinates
    1,487,038 1,487,514

    The protein

    Protein family

  • flavodoxin family (with [protein|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|YkuP], according to UniProt)

    • Paralogous protein(s)

  • [protein|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|YkuP]

    • [SW|Domains]

  • [SW|Flavodoxin-like domain] (aa 4-144) (according to UniProt)

    • [SW|Cofactors]

  • FMN [Pubmed|25194416]

    • Structure

  • [PDB|5LJL] (flavodoxin from Streptococcus pneumoniae, 42% identity) [pubmed|27649488]

    • Expression and Regulation

    Operons

    genes
    [gene|0932DDC285635C02067A3302CEDA302FE4269179|ykuN]-[gene|CEC1B5DF1E548597FE333895ECA476AB9DFFFDD7|ykuO]-[gene|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|ykuP]
    description
    [Pubmed|12354229]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12354229], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI]: sigma factor, [pubmed|29914988], in [regulon|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI regulon]

    • regulatory mechanism

  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: activation, [Pubmed|24214949], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]
  • [protein|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|Kre]: repression, in [regulon|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|Kre regulon]
  • [protein|EC6697D5D945B7E5083AFED9218748763C443278|NsrR]: repression, [Pubmed|24214949], in [regulon|EC6697D5D945B7E5083AFED9218748763C443278|NsrR regulon]
  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

    • regulation

  • expressed under anaerobic conditions ([protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]) [Pubmed|24214949]
  • induced by iron starvation (second wave) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [pubmed|29133393,12354229]
  • expression of the operon is strongly induced at the beginning of [SW|biofilm formation] [pubmed|31113899]

    • additional information

  • the ''[protein|0932DDC285635C02067A3302CEDA302FE4269179|YkuN]-[protein|CEC1B5DF1E548597FE333895ECA476AB9DFFFDD7|YkuO]-[protein|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|YkuP]'' operon is strongly unregulated in a ''[SW|kre]'' mutant [Pubmed|26110430]
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    Biological materials

    Mutant

  • MGNA-B337 (ykuN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1336 NBRP B. subtilis, Japan]
  • BKE14150 ([gene|0932DDC285635C02067A3302CEDA302FE4269179|ykuN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTATCACCCCATTAGT, downstream forward: _UP4_GATTATATGAACAAGGAAAA
  • BKK14150 ([gene|0932DDC285635C02067A3302CEDA302FE4269179|ykuN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTATCACCCCATTAGT, downstream forward: _UP4_GATTATATGAACAAGGAAAA

    • References

  • 15449930,17127770,12354229,20186410,24214949,21665975,25194416,25933252,26110430,28439033,27649488,29133393,29914988,31113899