SubtiBank SubtiBank
comFC
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comFC

late competence gene
locus
BSU_35450
pI
8.85
mw
26.26 kDa
protein length
229 aa Sequence Blast
gene length
690 bp Sequence Blast
function
compentence
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • Gene

    Coordinates
    3,641,125 3,641,814

    The protein

    Protein family

  • comF/gntX family (single member, according to UniProt)

    • Modification

  • phosphorylated on Arg-74 [Pubmed|22517742]

    • [SW|Localization]

  • localizes to one or both cell poles [Pubmed|21278288]

    • Expression and Regulation

    Operons

    genes
    [gene|45F27C0789199626BA90D2C69E8F13525C911478|comFA]-[gene|D290247A43280532947B707F3AF388D7A7F404D3|comFB]-[gene|04D9D70C0BCFDCBD00B5156908C348135080A9C2|comFC]-[gene|049B1EBF64F0EBC04CE6D529C8EDB0B6B2184DF5|yvyF]-[gene|F03144BF8A187C8931938A21433431B8961E8EE7|flgM]-[gene|6F4CD36D4C1EE99EF7A503F44F53AEB8EFFEAB7F|yvyG]-[gene|767ADBEF1B40CB3C74116CEA8CFB2FAF19C1FE7B|flgK]-[gene|82AB04023BCB57967C7501E6AEAC4BB593AA6480|flgL]-[gene|E5A59D2AF53FA220BA98D9653589A8F0D24F75B8|yviE]-[gene|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW]-[gene|93F328524989597C7D2329B25E665496C9631E87|csrA]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8412657], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|8045879], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

    • regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|8412657], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]
  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|21736639], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [Pubmed|19898538], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]
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    Biological materials

    Mutant

  • BKE35450 ([gene|04D9D70C0BCFDCBD00B5156908C348135080A9C2|comFC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCGAATCACATAATAAAC, downstream forward: _UP4_TAAATGATTCTGTTTTTATG
  • BKK35450 ([gene|04D9D70C0BCFDCBD00B5156908C348135080A9C2|comFC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCGAATCACATAATAAAC, downstream forward: _UP4_TAAATGATTCTGTTTTTATG

    • Expression vectors

  • for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

    • References

  • 11814663,8412657,21278288,22517742,28618091,30254116