endo-beta-1,3-1,4 glucanase
function
lichenan degradation
product
endo-beta-1,3-1,4 glucanase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates][category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins][SW|Categories] containing this gene/protein
[SW|utilization of specific carbon sources], [SW|sporulation proteins]This gene is a member of the following [SW|regulons]
[SW|CcpA regulon], [SW|LicT regulon]The protein
Catalyzed reaction/ biological activity
Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds (according to Swiss-Prot)Protein family
glycosyl hydrolase 16 family (according to Swiss-Prot)Structure
[PDB|3O5S][SW|Localization]
extracellular (signal peptide) [Pubmed|18957862]Expression and Regulation
Operon
''[protein|search|licT]-[protein|search|bglS]'' [Pubmed|8606172]''[protein|search|bglS]'' [Pubmed|8606172][SW|Sigma factor]
''[protein|search|licT]'': [protein|search|SigA] [Pubmed|8606172]Regulation
repressed by glucose (3.1-fold) ([protein|search|CcpA]) [Pubmed|12850135,8245831]expressed in the stationary phase (temporal activation) [Pubmed|8245830]weak induction by ß-glucosides ([protein|search|LicT]) [Pubmed|8606172]Regulatory mechanism
[protein|search|CcpA]: transcription repression [Pubmed|8245831][protein|search|LicT]: binding to an [SW|RNA switch] results in transcriptional antitermination [Pubmed|8606172]Biological materials
Mutant
GP427 (''[protein|search|licT]-[protein|search|bglS]'', erm), BGW7 (cat), both available in the [SW|Stülke] labReferences
18957862,12850135,6087283,8245830,8245831,8606172,23459129,24391357,24659048,25808979 
