SubtiBank SubtiBank
pdeH
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pdeH

c-di-GMP degrading phosphodiesterase
locus
BSU_31740
pI
5.36
mw
47.76 kDa
protein length
409 aa Sequence Blast
gene length
1230 bp Sequence Blast
function
degradation of c-di-GMP
product
c-di-GMP degrading phosphodiesterase
essential
no
ec
3.1.3.71
synonyms
yuxH,comB,yufA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.5|Additional chemotaxis signal transduction and regulatory proteins]

    • Gene

    Coordinates
    3,258,037 3,259,266

    Phenotypes of a mutant

  • about three-fold increase of intracellular levels of c-di-GMP [pubmed|31138629]

    • The protein

    Catalyzed reaction/ biological activity

  • degradation of c-di-GMP [Pubmed|23893111]

    • [SW|Domains]

  • contains an EAL domain [Pubmed|22821967]
  • EAL domain (aa 1-209) (according to UniProt)
  • HDOD domain (aa 203-392) (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|02628F274952F9AEEE9F2224B771A4CE4AAD1C8D|pdeH]
    description
    [Pubmed|2507523]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2507523], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647,15687200], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

    • regulation

  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647,15687200]
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    Biological materials

    Mutant

  • MGNA-A606 (yufA/yuxH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/606 NBRP B. subtilis, Japan]
  • GP1590 (kan) available in [SW|Jörg Stülke]'s lab
  • BKE31740 ([gene|02628F274952F9AEEE9F2224B771A4CE4AAD1C8D|pdeH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGATTTATCCCCCTTAC, downstream forward: _UP4_TATTTAGAGGCTCTGGAATG
  • BKK31740 ([gene|02628F274952F9AEEE9F2224B771A4CE4AAD1C8D|pdeH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGATTTATCCCCCTTAC, downstream forward: _UP4_TATTTAGAGGCTCTGGAATG

    • References

  • 14651647,15687200,2507523,22821967,23893111,31138629