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yobN

similar to L-amino acid oxidase

locus

BSU_19020

7.04

50.47 kDa

protein length

446 aa Sequence Blast

gene length

1341 bp Sequence Blast

function

unknown

product

unknown

essential

1.4.3.2

synonyms

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

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categories

[category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.8|Not yet assigned]

[category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates

2,074,439 2,075,779

The protein

Catalyzed reaction/ biological activity

L-α-amino acid + H₂O + O₂ --> 2-oxocarboxylate + H₂O₂ + NH₄⁺(according to UniProt)

Protein family

flavin monoamine oxidase family (single member, according to UniProt)

[SW|Cofactors]

FAD (according to UniProt)

Structure

[PDB|3KVE] (from Vipera ammodytes 35% identity)

Expression and Regulation

Operons

genes

[gene|02269068E6E4282A32BB5AE0FAE27F104202E5B5|yobN]-[gene|032110CBA492D54F6494984323D12D719EAEAAD9|yobO]

description

[Pubmed|22383849]

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Biological materials

Mutant

MGNA-A311 (yobN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/311 NBRP B. subtilis, Japan]

BKE19020 ([gene|02269068E6E4282A32BB5AE0FAE27F104202E5B5|yobN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTTGGAGGCACCTCT, downstream forward: _UP4_TAGATCATGCTCACATCTTG

BKK19020 ([gene|02269068E6E4282A32BB5AE0FAE27F104202E5B5|yobN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTTGGAGGCACCTCT, downstream forward: _UP4_TAGATCATGCTCACATCTTG