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gmuB [2018-06-22 17:46:55]
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gmuB [2018-06-22 17:46:55]

glucomannan-specific phosphotransferase system, EIIB component of the PTS
locus
BSU05810
pI
4.72
mw
11.30 kDa
protein length
103 aa Sequence Blast
gene length
309 bp Sequence Blast
function
glucomannan uptake and phosphorylation
product
glucomannan-specific phosphotransferase system EIIB component
essential
no
synonyms
ydhM
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.25|Utilization of glucomannan]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.3|Phosphorylation on a Cys residue]

    • Gene

    Coordinates
    626,622 → 626,933

    The protein

    Protein family

  • [protein|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|PtsI] permease, lactose permease (Lac) family [Pubmed|10627040]

    • Expression and Regulation

    Operons

    genes
    [gene|00A4A90685CED4AD46829C45718CF26F1D62F5D3|gmuB]-[gene|44014896157718D3A6190DFDBD663C5CB9DA63FC|gmuA]-[gene|76324E0A6CAA8E1DD0B0C6FAE1CAB402231BF7CC|gmuC]-[gene|D8C81A8768E9C6B47B5E196B54DC2A3A8256E7E6|gmuD]-[gene|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|gmuR]-[gene|EB6DE119E6FB18413DB2C2B62B114FB787164F74|gmuE]-[gene|35225776F6044513D3E77DBBD6A7A8FE976F506A|gmuF]-[gene|289ACC9D13BBFAA5C083EE525ECCC32DB06E075F|gmuG]
    description
    [Pubmed|18177310]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|18177310], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR]: repression, [Pubmed|18177310], in [regulon|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|18177310], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: activation, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

    • regulation

  • induced by cellobiose ([protein|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR]) [Pubmed|18177310]
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    Biological materials

    Mutant

  • MGNA-C188 (ydhM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2186 NBRP B. subtilis, Japan]
  • BKE05810 (Δ[gene|00A4A90685CED4AD46829C45718CF26F1D62F5D3|gmuB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTGCTATCCCCCCTGTT, downstream forward: _UP4_TTGTCCTTAATGGTGAATCA
  • BKK05810 (Δ[gene|00A4A90685CED4AD46829C45718CF26F1D62F5D3|gmuB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTGCTATCCCCCCTGTT, downstream forward: _UP4_TTGTCCTTAATGGTGAATCA

    • References

  • 18177310,10627040,22900538,20817675